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    <dc:description xml:lang="ja">Zfp277は、我々がポリコーム蛋白質Bmi1の結合因子としてYeast two-hybrid screeningにより単離した。Zfp277はポリコーム蛋白質と協調的にInk4a/Arf遺伝子の発現抑制に働き、Zfp277KO由来のマウス線維芽細胞はこの経路の異常によりInk4a/Arf遺伝子の発現が脱抑制し、早期の細胞老化が観察される。</dc:description>
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    <dc:description xml:lang="en">Zfp277 gene knockout mice. Exon 5 of Zfp277 gene was replaced with a floxed neo cassette. Homozygous mutant mice were born according to Menderian’s law, but the growth retardation and death of homozygous mice were sporadically observed.</dc:description>
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    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
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    <ns5:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;</ns5:addinfo>
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    <ns1:strainDevelopment xml:lang="ja">千葉大学 岩間 厚志先生 (2005年) R1 ES (Hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+&lt;p&gt;+&lt;Tyr-c&gt;Kitl&lt;Sl-J&gt;/+)</ns1:strainDevelopment>
    <ns1:ESCellLine>R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl&lt;+&gt;]</ns1:ESCellLine>
    <ns5:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr05541'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns5:addinfo>
    <skos:altLabel xml:lang="en">Zfp277 KO, B6-Zfp277 KO</skos:altLabel>
    <skos:altLabel xml:lang="ja">Zfp277 KO, B6-Zfp277 KO</skos:altLabel>
    <ns1:strainDevelopment xml:lang="en">Developed by Atsushi Iwama, Graduate School of Medicine, Chiba University in 2005. The mutant mice were backcrossed to C57BL/6N.</ns1:strainDevelopment>
    <ns0:brs_depositor xml:lang="en">Atsushi IWAMA</ns0:brs_depositor>
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    </ns1:references>
    <ns1:strainDevelopment xml:lang="ja">千葉大学 岩間 厚志先生 (2005年)R1 ES (Hybrid of two 129 substrains (129X1/SvJ and 129S1/SV-+&lt;p&gt;+&lt;Tyr-c&gt;Kitl&lt;Sl-J&gt;/+)</ns1:strainDevelopment>
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    <ns0:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_8"/>
    <ns5:termsAndConditions xml:lang="ja">条件を付加する。&lt;br&gt;研究成果の公表にあたって寄託者の指定する文献を引用する。PloS One., 5, e12373 (2010).</ns5:termsAndConditions>
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    <ns5:termsAndConditions xml:lang="en">In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PloS One., 5, e12373 (2010).</ns5:termsAndConditions>
    <ns1:genotypingProtocol rdf:resource="http://mus.brc.riken.jp/DataSheet/pcr05541"/>
    <ns1:colonyMaintenance xml:lang="ja">C57BL/6コンジェニック系統。C57BL/6NCr (日本SLC) に６世代以上の戻し交配を行う。その後，兄妹交配にて維持。</ns1:colonyMaintenance>
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    <ns1:donorDNA>Bacteriophage loxP sites, Mouse Phosphoglycerate kinase promoter (PGK promoter), E. coli Neomycin resistance gene, SV40 PolyA signal, mouse Zfp277 genomic DNA</ns1:donorDNA>
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    <ns7:SIO_001279 rdf:parseType="Resource">
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    <ns0:brs_depositor xml:lang="ja">岩間　厚志</ns0:brs_depositor>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/HealthCertificate_129039">
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